"Security operatives have made an attempt on the life of President @HEBobiwine. He was shot in the leg and seriously injured," Wine's party, the National Unity Platform, said in a post on the X platform.
Videos posted online showed Mr Wine surrounded by followers who shouted that he had been shot in the leg before some supported him into a waiting car.
Museveni's government has been accused by opponents and human rights activists of stifling the opposition, something Museveni denies.
Wine has amassed huge support amongst the youth in Uganda, a nation of 46 million, with many wooed initially by his rags-to-riches story as a pop star from the ghetto, and in recent years by his bold attacks on Museveni's government.
Bobi Wine tells Monitor that he will on Wednesday undergo surgery to remove canister fragments from his leg, per medics at the hospital. 
Wine was declared the runner-up in the 2021 presidential election, although he rejected the results and claimed to have won, saying the vote has been rigged through intimidation, ballot stuffing, bribery and other tactics.
The police say Bobi Wine had attended an event at Bulindo and afterwards "he and his team moved out of their cars and embarked on a procession up to Bulindo town.
Mr Wine ran for president in 2021, losing to President Yoweri Museveni in an election he claimed was rigged against him. His party, the National Unity Platform, holds the most seats of any opposition group in the national assembly.
The country's security forces have a long history of pursuing political opponents of President Museveni, who has been in power for almost 40 years.
Wine, a pop star turned politician whose real name is Robert Kyagulanyi, finished runner-up in the 2021 presidential election behind Museveni, who has ruled the East African country for nearly four decades.
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